STSM by Violaine VIZCAINO, CIMAP, Caen (FR) withThomas Schlatholter, Zernike Institute for Advanced Materials (NL)VVIZ_logos
On September 26, 2015 (10 days)
From FRANCE to NETHERLANDS

Radiation damage to human type II collagen fragments on the single molecule level

The purpose of the STSM was to study the radiation-induced molecular degradation of human type II collagen. We looked at the photo-fragmentation induced by both soft X-ray and XUV of two peptides: PK26-P [PGGPPGPKGNSGEPGAPGSKGDTGAK] which is made of a repetition of the amino acids sequence X-Y-Gly specific to collagen protein, and PK26-HyP [PGGP-HyP-GPKGNSGE-HyP-GA-HyP-GSKGDTGAK] which contains the unnatural hydroxyproline (HyP) residue.

Fragmentation of both peptides, in two different protonated states (M+3H)3+ and (M+4H)4+, were measured at different photon energies in order to highlight the main fragmentation channels, their threshold and the influence of hydroxyproline (HyP) residue in these processes. Figure 1 shows typical fragmentation spectra of (PK26-Hyp +4H)4+ at three different energies (14, 18 and 21 eV).

We expect to submit our results in a peer-reviewed scientific journal at the beginning of 2016. Moreover, this STSM has also allowed strengthening the collaboration between the CIMAP (Caen, France) and the University of Groningen (Netherlands).

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Figure 1 : Fragmentation spectra of (PK26-Hyp +4H)4+. All spectra are normalized to the parent depletion.

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